The Science
Decoding how you age
What is biological age?
Biological age is calculated using advanced algorithms that analyse multiple blood biomarkers reflecting your body's functional health. It goes beyond chronological age to reveal how your cells, organs and systems are truly aging.
Every major chronic condition of modern life is, at root, linked to accelerated biological ageing.
Why blood?
A single standard venous blood draw captures metabolic, hormonal, inflammatory, nutritional and organ-function signals at once. No genetic test, no wearable required. Objective, repeatable, and re-testable over time so you can see change.
Proprietary analytics
The Holistica Biological Age Engine
The Engine brings multiple blood biomarkers and physiological domains together into a more meaningful picture of your aging pattern. It decomposes a single blood draw into nine independently scored organ systems and expresses each one as the number of years it adds to, or subtracts from, your biological age.
Built with input from internal medicine, endocrinology, gastroenterology, cardiology, integrative and longevity physicians, and calibrated with Indian and South Asian population data in mind.
Methodology
Our five assessment pillars
Biochemical Biomarkers
91 key blood parameters covering metabolic, hormonal, inflammatory, nutritional and organ health.
Cellular Health
Markers reflecting oxidative stress, inflammation, immune function and cellular energy.
Organ-System Function
How well each organ system is performing.
Lifestyle & Risk Factors
Daily habits, environmental exposures and personal health risks.
Data Science & Longevity Algorithm
Proprietary algorithms integrate all data points to calculate your Biological Age and Age Gap.
Nine systems
The organ systems we score
Our philosophy
Diagnose before you prescribe
Every recommendation is tied to a measured marker. Each marker is placed in a 5-zone analysis, from optimal to high risk. The aging drivers, the systems adding years, are prioritised by expected impact, and the protective biology already working in your favour is preserved.
Interventions span nutrition, movement, sleep, recovery, supplements and therapies, and integrate modern medicine with natural, preventive and Indian traditional approaches under physician guidance.
Marker explorer
Know your markers
| Marker | System | Reference range | What it means |
|---|---|---|---|
| HbA1c | Glucose & Metabolic | 4.2–5.7 % | Your average blood sugar over roughly three months. |
| Fasting glucose | Glucose & Metabolic | 70–100 mg/dL | Blood sugar after an overnight fast. |
| C-peptide | Glucose & Metabolic | 0.48–5.05 ng/mL | Reflects how much insulin your pancreas is producing. |
| Total cholesterol | Lipid & Cardio | <200 mg/dL | The total amount of cholesterol carried in your blood. |
| LDL cholesterol | Lipid & Cardio | <100 mg/dL | Cholesterol that can build up in artery walls. |
| HDL cholesterol | Lipid & Cardio | 40–60 mg/dL | Cholesterol that helps clear excess lipids. |
| Triglycerides | Lipid & Cardio | <150 mg/dL | Circulating fats, sensitive to sugar, alcohol and refined carbohydrates. |
| Non-HDL cholesterol | Lipid & Cardio | <160 mg/dL | All potentially artery-clogging cholesterol combined. |
| Homocysteine | Lipid & Cardio | 3.7–13.9 µmol/L | An amino acid linked to vascular health and B-vitamin status. |
| hs-CRP | Inflammation | <5 mg/L | A sensitive marker of low-grade systemic inflammation. |
| ESR | Inflammation | 0–12 mm/hr | A general, non-specific indicator of inflammation. |
| AST | Liver | 3–35 U/L | An enzyme released when liver or muscle cells are stressed. |
| ALT | Liver | 3–35 U/L | A liver-specific enzyme, often raised with fatty liver. |
| ALP | Liver | 33–98 U/L | An enzyme from the liver, bile ducts and bone. |
| GGT | Liver | 5–38 U/L | Sensitive to alcohol and oxidative stress in the liver. |
| Bilirubin | Liver | 0.3–1.2 mg/dL | A by-product of red blood cell breakdown processed by the liver. |
| Creatinine | Kidney | 0.4–0.9 mg/dL (F) | A muscle waste product cleared by the kidneys. |
| eGFR | Kidney | ≥90 mL/min/1.73m² | An estimate of how well your kidneys filter blood. |
| BUN | Kidney | 8–20 mg/dL | Nitrogen from protein breakdown, cleared by the kidneys. |
| Urea | Kidney | 17–43 mg/dL | Waste from protein metabolism. |
| Uric acid | Kidney | 2.6–6.0 mg/dL | Linked to purine intake, metabolic health and gout risk. |
| Total protein | Protein & Nutrition | 6.6–8.3 g/dL | All proteins in your blood combined. |
| Albumin | Protein & Nutrition | 3.5–5.2 g/dL | A key marker of nutritional reserve and liver function. |
| Globulin | Protein & Nutrition | 3.0–4.2 g/dL | Proteins involved in immunity and transport. |
| Haemoglobin | Haemogram | 12–15 g/dL (F) | The oxygen-carrying protein in red blood cells. |
| Haematocrit | Haemogram | 36–46 % (F) | The proportion of blood made up of red cells. |
| RBC count | Haemogram | 3.8–4.8 million/µL | The number of red blood cells. |
| MCV | Haemogram | 83–101 fL | The average size of your red blood cells. |
| RDW-CV | Haemogram | 11.6–14 % | Variation in red cell size, an emerging ageing signal. |
| Platelets | Haemogram | 150–410 ×10³/µL | Cells involved in clotting. |
| MPV | Haemogram | 7–9 fL | The average size of your platelets. |
| WBC | Immune | 4–10 ×10³/µL | Total white blood cells, your immune workforce. |
| Neutrophils | Immune | 40–80 % | First-responder immune cells. |
| Lymphocytes | Immune | 20–40 % | Adaptive immune cells, which tend to decline with age. |
| Calcium | Minerals & Hormonal | 8.8–10.6 mg/dL | Essential for bones, muscles and nerve signalling. |
| Phosphorus | Minerals & Hormonal | 2.5–4.5 mg/dL | Works with calcium for bone and energy metabolism. |
| Magnesium | Minerals & Hormonal | 1.9–2.5 mg/dL | Supports hundreds of enzymes, sleep and muscle function. |
| Sodium | Minerals & Hormonal | 136–146 mmol/L | Key for fluid balance and nerve function. |
| Potassium | Minerals & Hormonal | 3.5–5.5 mmol/L | Essential for heart rhythm and muscle function. |
| Chloride | Minerals & Hormonal | 101–109 mmol/L | Helps maintain fluid and acid-base balance. |
| Vitamin D3 | Minerals & Hormonal | 30–100 ng/mL | Supports bone, immunity and muscle health. |
| Vitamin B12 | Minerals & Hormonal | 211–912 pg/mL | Needed for nerves, red cells and energy. |
| TSH | Minerals & Hormonal | 0.55–4.78 µIU/mL | The pituitary signal that regulates your thyroid. |
| T3 | Minerals & Hormonal | 0.60–1.81 ng/mL | The active thyroid hormone. |
| T4 | Minerals & Hormonal | 3.2–12.6 µg/dL | The main hormone produced by the thyroid. |
Reference ranges vary by lab, age and sex. Your report uses your lab's ranges. Consult your physician for medical advice.
Biological age is dynamic
Consistent improvements in diet, exercise, sleep and stress management can reduce biological age by 1 to 5 years over 12 to 24 months. Your report shows you where to begin.
Book Your Test“What gets measured can be understood. What is understood can be changed.”